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Cardiac Troponin I antibody [N1C3-3]

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028
Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028
Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028
Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028

Cat. No. GTX113028

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Pig
Package
100 μl ($399),
25 μl ($169)
See all Cardiac Troponin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

Raji , mouse heart

Predict Reactivity

Rat, Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cardiac Troponin I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722789

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028

GTX113028 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX113028 diluted at 1:1000

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028

GTX113028 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX113028 diluted at 1:5000

Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028

GTX113028 ICC/IF Image

Cardiac Troponin I antibody [N1C3-3] detects Cardiac Troponin I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cardiac Troponin I protein stained by Cardiac Troponin I antibody [N1C3-3] (GTX113028) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028

GTX113028 ICC/IF Image

The data was published in the journal PLoS One in 2015. PMID: 25680105

REFERENCE

Application Reference

Intawicha P et al. Theriogenology 2016; (Epub) Derivation and characterization of putative embryonic stem cells from cloned rabbit embryos.
Application : ICC/IF
Siriboon C et al. PLoS One 2015; 10 (2) : e0118165 Putative porcine embryonic stem cell lines derived from aggregated four-celled cloned embryos produced by oocyte bisection cloning.
Application : IHC
Reactivity : Pig
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169