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Cardiac Troponin I antibody [N1C3-3]

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028
Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028
Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028

Cat No. GTX113028

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC

Reactivity

Human, Mouse, Pig
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , Raji , mouse heart

Predict Reactivity

Rat, Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cardiac Troponin I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722789

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028

GTX113028 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX113028 diluted at 1:1000

Anti-Cardiac Troponin I antibody [N1C3-3] used in Western Blot (WB). GTX113028

GTX113028 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX113028 diluted at 1:5000

Anti-Cardiac Troponin I antibody [N1C3-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113028

GTX113028 ICC/IF Image

Cardiac Troponin I antibody [N1C3-3] detects Cardiac Troponin I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cardiac Troponin I protein stained by Cardiac Troponin I antibody [N1C3-3] (GTX113028) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

Cardiac Troponin I antibody [N1C3-3] Cat No. GTX113028

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Pig
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 25680105
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169