Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I (phospho Ser23/Ser24) antibody (GTX133976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I (phospho Ser23/Ser24) antibody (GTX133976) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.