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Cardiac Troponin T antibody

Comparison Orthogonal
Anti-Cardiac Troponin T antibody used in Western Blot (WB). GTX134489
Anti-Cardiac Troponin T antibody used in Western Blot (WB). GTX134489
Anti-Cardiac Troponin T antibody used in IHC (Paraffin sections) (IHC-P). GTX134489
Anti-Cardiac Troponin T antibody used in Western Blot (WB). GTX134489
Anti-Cardiac Troponin T antibody used in Western Blot (WB). GTX134489
Anti-Cardiac Troponin T antibody used in IHC (Paraffin sections) (IHC-P). GTX134489

Cat. No. GTX134489

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cardiac Troponin T products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

mouse heart , rat heart

Predict Reactivity

Cat, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Cardiac Troponin T. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887294

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

troponin T2, cardiac type , CMD1D , CMH2 , CMPD2 , LVNC6 , RCM3 , TnTC , cTnT

Background

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cardiac Troponin T antibody used in Western Blot (WB). GTX134489

GTX134489 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (GTX134489) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Cardiac Troponin T antibody used in Western Blot (WB). GTX134489

GTX134489 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (GTX134489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin T antibody used in IHC (Paraffin sections) (IHC-P). GTX134489

GTX134489 IHC-P Image

Cardiac Troponin T antibody detects Cardiac Troponin T protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Cardiac Troponin T stained by Cardiac Troponin T antibody (GTX134489) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cardiac Troponin T antibody used in Western Blot (WB). GTX134489

GTX134489 WB Image

Human heart (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (GTX134489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin T antibody used in Western Blot (WB). GTX134489

GTX134489 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (GTX134489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cardiac Troponin T antibody used in IHC (Paraffin sections) (IHC-P). GTX134489

GTX134489 IHC-P Image

Cardiac Troponin T antibody detects Cardiac Troponin T protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Cardiac Troponin T stained by Cardiac Troponin T antibody (GTX134489) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Garc?a-Arroyo FE et al. Antioxidants (Basel) 2023; 12 (5) Osthole Prevents Heart Damage Induced by Diet-Induced Metabolic Syndrome: Role of Fructokinase (KHK).
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169