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Cas9 antibody [7A9]

Anti-Cas9 antibody [7A9] used in Immunoprecipitation (IP). GTX53807
Anti-Cas9 antibody [7A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53807

Cat No. GTX53807

Host

Mouse

Clonality

Monoclonal

Clone Name

7A9

Isotype

IgG1

Application

WB, ICC/IF, IP
Package
25 μg ($149)

APPLICATION

Application Note

Western Blotting Analysis: 0.5 μg/mL from a representative lot detected Cas9 in 1 μg of Myc-Cas9 expressing HEK293 cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).

Specificity/Sensitivity

This antibody recognises both Cas9 and dCas9 (nuclease deficient Cas9)

PROPERTIES

Form

Liquid

Buffer

Buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

N-Terminal domain of Cas9.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Cas9 antibody [7A9] used in Immunoprecipitation (IP). GTX53807

GTX53807 IP Image

IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.

Anti-Cas9 antibody [7A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53807

GTX53807 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 0.5 μg/ml (green) and DAPI (blue).

REFERENCE

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Package List Price ($)
$ 149