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Casein Kinase 1 alpha 1L antibody

Anti-Casein Kinase 1 alpha 1L antibody used in IHC (Whole mount) (IHC-Wm). GTX105584
Anti-Casein Kinase 1 alpha 1L antibody used in Western Blot (WB). GTX105584
Anti-Casein Kinase 1 alpha 1L antibody used in IHC (Paraffin sections) (IHC-P). GTX105584
Anti-Casein Kinase 1 alpha 1L antibody used in Western Blot (WB). GTX105584
Anti-Casein Kinase 1 alpha 1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105584

Cat. No. GTX105584

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Casein Kinase 1 alpha 1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

A549 , rat thymus

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Casein Kinase 1 alpha 1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162808

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

casein kinase 1 alpha 1 like , CK1

Cellular Localization

Cytoplasm

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling.

Database

Research Area

DATA IMAGES

Anti-Casein Kinase 1 alpha 1L antibody used in IHC (Whole mount) (IHC-Wm). GTX105584

GTX105584 IHC-Wm Image

Immunofluorescent image of a cross-section of a zebrafish embryo using Casein Kinase 1 alpha 1L antibody (GTX105584) at a 1:200 dilution. Casein Kinase 1 alpha 1L is localized in neurons in the spinal cord. Casein Kinase 1 alpha 1L (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-Casein Kinase 1 alpha 1L antibody used in Western Blot (WB). GTX105584

GTX105584 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX105584 diluted at 1:500

Anti-Casein Kinase 1 alpha 1L antibody used in IHC (Paraffin sections) (IHC-P). GTX105584

GTX105584 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Casein Kinase 1 alpha 1L(GTX105584) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Casein Kinase 1 alpha 1L antibody used in Western Blot (WB). GTX105584

GTX105584 WB Image

Casein Kinase 1 alpha 1L antibody detects CSNK1A1L protein by Western blot analysis.
A. 50 μg rat thymus lysate/extract
10 % SDS-PAGE
Casein Kinase 1 alpha 1L antibody (GTX105584) dilution: 1:500

Anti-Casein Kinase 1 alpha 1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105584

GTX105584 ICC/IF Image

Casein Kinase 1 alpha 1L antibody detects CSNK1A1L protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CSNK1A1L protein stained by Casein Kinase 1 alpha 1L antibody (GTX105584) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169