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Casein Kinase 1 alpha antibody [AT2E2]

Anti-Casein Kinase 1 alpha antibody [AT2E2] used in Western Blot (WB). GTX50020

Cat. No. GTX50020

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2E2

Isotype

 IgG2b

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Casein Kinase 1 alpha products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1000 ~ 2000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

RRID

AB_11174839

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

casein kinase 1 alpha 1 , CK1 , CK1a , CKIa , HEL-S-77p , HLCDGP1 , PRO2975

Cellular Localization

Cytoplasm , Nucleus speckle

Database

Research Area

DATA IMAGES

Anti-Casein Kinase 1 alpha antibody [AT2E2] used in Western Blot (WB). GTX50020

GTX50020 WB Image

Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detectio

REFERENCE

Application Reference

Iaconelli J et al. ACS Chem Biol 2015; 10 (3) : 883-90 HDAC6 Inhibitors Modulate Lys49 Acetylation and Membrane Localization of β-Catenin in Human iPSC-Derived Neuronal Cells.
Application : WB
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399