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Casein Kinase 1 alpha antibody [AT2E2]

Anti-Casein Kinase 1 alpha antibody [AT2E2] used in Western Blot (WB). GTX50020

Cat No. GTX50020

Host

Mouse

Clonality

Monoclonal

Clone Name

AT2E2

Isotype

IgG2b

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1000 ~ 2000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Casein Kinase 1 Alpha 1 , Ck1 , Ck1A , Ckia , Hel-S-77P , Hlcdgp1 , Pro2975 , Csnk1A1

Cellular Localization

Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.

Background

Caseine Kinase 1 alpha belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily. CK1 isoforms (alpha, beta, gamma, delta, epsilon) and their splice variants are involved in diverse cellular processes including membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Perhaps it can phosphorylate a large number of proteins and is involved in Wnt signaling where it phosphorylates CTNNB1 on Ser45. Caseine kinase 1 alpha also interacts with the Axin complex

Database

Research Area

DATA IMAGES

Anti-Casein Kinase 1 alpha antibody [AT2E2] used in Western Blot (WB). GTX50020

GTX50020 WB Image

Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detectio

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf