GeneTex
United States (US)

Casein Kinase 1 alpha antibody

CKI-alpha-antibody-GTX87999-WB-1_18121409_311.jpg
CKI-alpha-antibody-GTX87999-ICC_IF-1_18121409_370.jpg

Cat No. GTX87999

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Specificity/Sensitivity

CKI-alpha (Ab-321) Antibody detects endogenous levels of total Casein Kinase 1 alpha protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Casein Kinase 1 alpha.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

kinase CK1-alpha, EC2.7.11.1, KC1A, kinase CK1alpha, Casein kinase I, CKI-alpha, CKIalpha, CK1alpha, CKI alpha, CK1-alpha, alpha isoform, CSNK1A1, EC 2.7.11.1, CK1 alpha, kinase CK1 alpha

Cellular Localization

Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
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Database

Research Area

DATA IMAGES
CKI-alpha-antibody-GTX87999-WB-1_18121409_311.jpg

GTX87999 WB Image

Western blot analysis of extracts from HuvEc/K562 cells, using CKI-alpha (Ab-321) Antibody. The lane on the right is treated with the synthesized peptide.

CKI-alpha-antibody-GTX87999-ICC_IF-1_18121409_370.jpg

GTX87999 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using CKI-alpha (Ab-321) Antibody. The picture on the right is treated with the synthesized peptide.