GeneTex
United States (US)

Casein Kinase 1 alpha antibody

CKI-alpha-antibody-GTX88000-IHC-1_18121409_142.jpg
CKI-alpha-antibody-GTX88000-ICC_IF-1_18121409_363.jpg
CKI-alpha-antibody-GTX88000-WB-1_18121409_175.jpg

Cat No. GTX88000

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

38 kDa. ( Note )

Specificity/Sensitivity

CKI-alpha Antibody detects endogenous levels of total Casein Kinase 1 alpha protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Casein Kinase 1 alpha.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Casein Kinase 1 Alpha 1,Ck1,Ck1A,Ckia,Hel-S-77P,Hlcdgp1,Pro2975,Csnk1A1

Cellular Localization

Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Database

Research Area

DATA IMAGES
CKI-alpha-antibody-GTX88000-IHC-1_18121409_142.jpg

GTX88000 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CKI-alpha Antibody. The picture on the right is treated with the synthesized peptide.

CKI-alpha-antibody-GTX88000-ICC_IF-1_18121409_363.jpg

GTX88000 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using CKI-alpha Antibody. The picture on the right is treated with the synthesized peptide.

CKI-alpha-antibody-GTX88000-WB-1_18121409_175.jpg

GTX88000 WB Image

Western blot analysis of extracts from HeLa cells, using CKI-alpha Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339