GeneTex
United States (US)

Casein Kinase 2 alpha antibody

GTX107576_40366_WB_19011506_912.jpg
GTX107576_40366_WB_M_19011506_968.jpg
GTX107576_40366_IHC_19011506_938.jpg

Cat No. GTX107576

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CK2A1 antibody, CKII antibody, CSNK2A1 antibody, casein kinase II alpha 1 subunit antibody, CK II alpha antibody, protein kinase CK2 antibody, CK2 catalytic subunit alpha antibody, casein kinase II subunit alpha antibody, "casein kinase 2, alpha 1 polypeptide antibody"

Background

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq]
DATA IMAGES
GTX107576_40366_WB_19011506_912.jpg

GTX107576 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX107576 diluted at 1:1000

GTX107576_40366_WB_M_19011506_968.jpg

GTX107576 WB Image

Sample (30 ug of whole cell lysate)
B: JC
C: BCL-1
10% SDS PAGE
GTX107576 diluted at 1:1000

GTX107576_40366_IHC_19011506_938.jpg

GTX107576 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using Casein Kinase 2 alpha 1(GTX107576) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169