Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Casein Kinase 2 alpha antibody detects CSNK2A1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSNK2A1 protein stained by Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Casein Kinase 2 alpha 1(GTX107897) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Casein Kinase 2 alpha antibody detects CSNK2A1 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10% SDS-PAGE
Casein Kinase 2 alpha antibody (GTX107897) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

The data was published in the journal Oncogene in 2017. PMID: 28436950