GeneTex
United States (US)

Casein Kinase 2 alpha antibody

GTX107897_WB_19011507_928.jpg
GTX107897_40002_IFA_19011507_554.jpg
GTX107897_40002_20160526_WB_R_19011507_741.jpg
GTX107897_40002_IHC_19011507_993.jpg
GTX107897_40002_WB_M_brain_19011507_580.jpg
GTX107897_40002_20160526_WB_R_brain_19011507_536.jpg

Cat No. GTX107897

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , PC-12 , Rat2 , rat brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CK2A1 antibody, CKII antibody, CSNK2A1 antibody, casein kinase II alpha 1 subunit antibody, CK II alpha antibody, protein kinase CK2 antibody, CK2 catalytic subunit alpha antibody, casein kinase II subunit alpha antibody, casein kinase 2, alpha 1 polypeptide antibody

Background

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq]
DATA IMAGES
GTX107897_WB_19011507_928.jpg

GTX107897 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX107897 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107897_40002_IFA_19011507_554.jpg

GTX107897 ICC/IF Image

Casein Kinase 2 alpha antibody detects CSNK2A1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSNK2A1 protein stained by Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX107897_40002_20160526_WB_R_19011507_741.jpg

GTX107897 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107897_40002_IHC_19011507_993.jpg

GTX107897 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Casein Kinase 2 alpha 1(GTX107897) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107897_40002_WB_M_brain_19011507_580.jpg

GTX107897 WB Image

Casein Kinase 2 alpha antibody detects CSNK2A1 protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
10% SDS-PAGE
Casein Kinase 2 alpha antibody (GTX107897) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107897_40002_20160526_WB_R_brain_19011507_536.jpg

GTX107897 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (GTX107897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169