GeneTex
United States (US)

Casein Kinase 2 beta antibody

GTX101439_39645_IHC_19011506_778.jpg
GTX101439_39645_WB_M_19011506_429.jpg
GTX101439_39645_IHC_M_19011506_316.jpg
GTX101439_39645_IFA_19011506_208.jpg
GTX101439_WB_19011506_905.jpg

Cat No. GTX101439

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

MCF-7 , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Rabbit, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Casein Kinase 2 Beta,Ck2B,Ck2N,Csk2B,Ckb1,Ckb2,G5A,Csnk2B

Background

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101439_39645_IHC_19011506_778.jpg

GTX101439 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Casein Kinase 2 beta(GTX101439) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101439_39645_WB_M_19011506_429.jpg

GTX101439 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX101439 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101439_39645_IHC_M_19011506_316.jpg

GTX101439 IHC-P Image

Casein Kinase 2 beta antibody detects Casein Kinase 2 beta protein at nucleus and cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Casein Kinase 2 beta antibody (GTX101439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101439_39645_IFA_19011506_208.jpg

GTX101439 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Casein Kinase 2 beta(GTX101439) antibody at 1:500 dilution.

GTX101439_WB_19011506_905.jpg

GTX101439 WB Image

Sample (30 µg of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX101439 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf