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Casein Kinase 2 beta antibody

Anti-Casein Kinase 2 beta antibody used in Western Blot (WB). GTX101439
Anti-Casein Kinase 2 beta antibody used in Western Blot (WB). GTX101439
Anti-Casein Kinase 2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101439
Anti-Casein Kinase 2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101439
Anti-Casein Kinase 2 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101439

Cat No. GTX101439

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

MCF-7 , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Rabbit, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

casein kinase 2 beta,CK2B,CK2N,CSK2B,Ckb1,Ckb2,G5A

Background

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Casein Kinase 2 beta antibody used in Western Blot (WB). GTX101439

GTX101439 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 beta antibody (GTX101439) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Casein Kinase 2 beta antibody used in Western Blot (WB). GTX101439

GTX101439 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 beta antibody (GTX101439) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Casein Kinase 2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101439

GTX101439 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Casein Kinase 2 beta(GTX101439) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Casein Kinase 2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101439

GTX101439 IHC-P Image

Casein Kinase 2 beta antibody detects Casein Kinase 2 beta protein at nucleus and cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Casein Kinase 2 beta antibody (GTX101439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Casein Kinase 2 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101439

GTX101439 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Casein Kinase 2 beta(GTX101439) antibody at 1:500 dilution.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169