APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG
Predict Reactivity
Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10724304
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
casein kinase 2 beta , CK2B , CK2N , CSK2B , Ckb1 , Ckb2 , G5A
Background
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113335 WB Image
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE GTX113335 diluted at 1:500
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GTX113335 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Casein Kinase 2 beta(GTX113335) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113335 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Casein Kinase 2 beta(GTX113335) antibody at 1:500 dilution.
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REFERENCE
There are currently no references for Casein Kinase 2 beta antibody (GTX113335). Be the first to share your publications with this product.
REVIEW
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