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Casein Kinase 2 beta (phospho Ser209) antibody

Anti-Casein Kinase 2 beta (phospho Ser209) antibody used in Western Blot (WB). GTX12861
Anti-Casein Kinase 2 beta (phospho Ser209) antibody used in Western Blot (WB). GTX12861

Cat No. GTX12861

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, FACS
Reactivity Human
Package
50 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Specificity/Sensitivity

Does not cross react with Casein Kinase 2 alpha [pS₃₆] (mouse), [pS₃₆₂], [pS₃₇₀], or Casein Kinase 2 beta pS₂].
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human CK2â that contains serine 209. The sequence is conserved in mouse, rat, chicken, pig and rabbit.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Casein Kinase 2 Beta,Ck2B,Ck2N,Csk2B,Ckb1,Ckb2,G5A,Csnk2B

Background

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES
Anti-Casein Kinase 2 beta (phospho Ser209) antibody used in Western Blot (WB). GTX12861

GTX12861 WB Image

WB analysis of whole cell extract (30ug) of Jurkat (Lane 1), K562 (Lane 2), KARPAS 299 (Lane 3), HEL 92.1.7 (Lane 4), THP1 (Lane 5), A431 (Lane 6) and A549 (Lane 7) using GTX12861 Casein Kinase 2 beta (phospho Ser209) antibody.
Dilution : 1:250

Anti-Casein Kinase 2 beta (phospho Ser209) antibody used in Western Blot (WB). GTX12861

GTX12861 WB Image

WB analysis of HeLa cells using GTX12861 Casein Kinase 2 beta (phospho Ser209) antibody.
The primary antibody was prior incubated with different peptide (Lane 1~4).
The membrane treated with lambda phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE
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Package List Price ($)
$ 319