GeneTex
United States (US)

Caspase 1 antibody [14F468]

GTX14367_1078_WB_18121410_640.jpg
GTX14367_446_IHC-P_18121410_694.jpg
GTX14367_444_IHC-P_18121410_577.jpg
GTX14367_445_IHC-P_18121410_763.jpg
GTX14367_447_IHC-P_18121410_380.jpg
GTX14367_448_IHC-P_18121410_862.jpg
GTX14367_1077_WB_18121410_189.jpg

Cat No. GTX14367

Host Mouse
Clonality Monoclonal
Clone Name 14F468
Isotype IgG1
Application WB, IHC-P, FACS
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/ml
IHC-P 1:10 - 1:500
FACS Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Specificity/Sensitivity

The antibody will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.

Predict Reactivity

Pig, Horse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 1,Ice,Il1Bc,P45,Casp1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX14367_1078_WB_18121410_640.jpg

GTX14367 WB Image

WB analysis of HeLa cell lysate using GTX14367 Caspase 1 antibody [14F468].

GTX14367_446_IHC-P_18121410_694.jpg

GTX14367 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX14367 Caspase 1 antibody [14F468].
Dilution : 5 μg/ml

GTX14367_444_IHC-P_18121410_577.jpg

GTX14367 IHC-P Image

IHC-P analysis of human colon tissue using GTX14367 Caspase 1 antibody [14F468].
Dilution : 5 μg/ml

GTX14367_445_IHC-P_18121410_763.jpg

GTX14367 IHC-P Image

IHC-P analysis of human lung tissue using GTX14367 Caspase 1 antibody [14F468].
Dilution : 5 μg/ml

GTX14367_447_IHC-P_18121410_380.jpg

GTX14367 IHC-P Image

IHC-P analysis of human rectum adenocarcinoma tissue using GTX14367 Caspase 1 antibody [14F468].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

GTX14367_448_IHC-P_18121410_862.jpg

GTX14367 IHC-P Image

IHC-P analysis of human skin tissue using GTX14367 Caspase 1 antibody [14F468].
Dilution : 5 μg/ml

GTX14367_1077_WB_18121410_189.jpg

GTX14367 WB Image

WB analysis of HeLa (A) and NIH-3T3 (B) cell lysate using GTX14367 Caspase 1 antibody [14F468].
Dilution : 0.5 μg/ml and 2 μg/ml

REFERENCE