GeneTex
United States (US)

Caspase 1 antibody [N1N3]

GTX101322_40184_20160728_WB_LPS_ATP_19011506_105.jpg
GTX101322_40457_IFA_19011506_551.jpg
GTX101322_40184_WB_19011506_368.jpg
GTX101322_40184_IHC_19011506_468.jpg

Cat No. GTX101322

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , THP-1 conditioned medium (500 ng/ml LPS treatment for 4 hr and 1 mM ATP treatment for 1.5hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 1,Ice,Il1Bc,P45,Casp1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101322_40184_20160728_WB_LPS_ATP_19011506_105.jpg

GTX101322 WB Image

Treated (+) THP-1 conditioned medium (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:1000.

GTX101322_40457_IFA_19011506_551.jpg

GTX101322 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 alpha(GTX101322) antibody at 1:500 dilution.

GTX101322_40184_WB_19011506_368.jpg

GTX101322 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX101322 diluted at 1:5000

GTX101322_40184_IHC_19011506_468.jpg

GTX101322 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha(GTX101322) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf