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Caspase 1 antibody [N1N3]

Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322
Anti-Caspase 1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101322
Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322
Anti-Caspase 1 antibody [N1N3] used in Western Blot (WB). GTX101322

Cat No. GTX101322

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

THP-1

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618781

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 1 , ICE , IL1BC , P45

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322

GTX101322 IHC-P Image

Caspase 1 antibody [N1N3] detects Caspase 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Caspase 1 stained by Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101322

GTX101322 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 alpha(GTX101322) antibody at 1:500 dilution.

Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322

GTX101322 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha(GTX101322) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caspase 1 antibody [N1N3] used in Western Blot (WB). GTX101322

GTX101322 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Caspase 1 antibody [N1N3] Cat No. GTX101322

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23653660
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23653660
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169