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Caspase 1 antibody

Orthogonal
Anti-Caspase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111630
Anti-Caspase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111630
Anti-Caspase 1 antibody used in Western Blot (WB). GTX111630
Anti-Caspase 1 antibody used in Western Blot (WB). GTX111630

Cat. No. GTX111630

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Caspase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A549

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620195

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 1 , ICE , IL1BC , P45

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111630

GTX111630 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 beta(GTX111630) antibody at 1:500 dilution.

Anti-Caspase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111630

GTX111630 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Caspase 1(GTX111630) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caspase 1 antibody used in Western Blot (WB). GTX111630

GTX111630 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody (GTX111630) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Caspase 1 antibody used in Western Blot (WB). GTX111630

GTX111630 WB Image

Orthogonal

The data was published in the journal Front Cell Infect Microbiol in 2018.PMID: 30186771

REFERENCE

Application Reference

Qiao L et al. J Diabetes Investig 2024; 15 (10) : 1365-1376 Calpeptin improves the cognitive function in Alzheimer's disease-like complications of diabetes mellitus rats by regulating TXNIP/NLRP3 inflammasome.
Wei KC et al. Front Cell Infect Microbiol 2018; 8 : 268 Dengue Virus Infects Primary Human Hair Follicle Dermal Papilla Cells.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169