GeneTex
United States (US)

Caspase 1 antibody

GTX14368_1079_WB_18121410_576.jpg
GTX14368_449_IHC-P_18121410_425.jpg

Cat No. GTX14368

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/ml
IHC-P 5 μg/ml
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Specificity/Sensitivity

The antibody recognizes full-length Caspase-1. The antibody also recognizes cleaved Caspase-1 forms that include amino acids 31-45.

Predict Reactivity

Rabbit, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human Caspase-1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 1,Ice,Il1Bc,P45,Casp1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX14368_1079_WB_18121410_576.jpg

GTX14368 WB Image

WB analysis of HeLa cell lysate using GTX14368 Caspase 1 antibody.
Dilution : 0.5μg/ml

GTX14368_449_IHC-P_18121410_425.jpg

GTX14368 IHC-P Image

IHC-P analysis of human reactive lymph node tissue using GTX14368 Caspase 1 antibody. B is a higher magnification of A. Staining in macrophages is observed.