GeneTex
United States (US)

Caspase 1 antibody

GTX31702_2868_WB_20180221_18121410_512.jpg
GTX31702_1352_IHC-P_20180221_18121410_879.jpg

Cat No. GTX31702

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide

Specificity/Sensitivity

Depending on cell lines or tissues used, other cleavage products may be observed.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Caspase-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-1.The immunogen is located within the last 50 amino acids of Caspase-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 1,Ice,Il1Bc,P45,Casp1

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES
GTX31702_2868_WB_20180221_18121410_512.jpg

GTX31702 WB Image

WB analysis of human heart tissue lysate using GTX31702 Caspase 1 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

GTX31702_1352_IHC-P_20180221_18121410_879.jpg

GTX31702 IHC-P Image

IHC-P analysis of human heart tissue using GTX31702 Caspase 1 antibody.
Working concentration : 2 μg/ml