GeneTex
United States (US)

Caspase 1 (cleaved Asn120) antibody

CASP1-cleaved-Asn120-antibody-GTX86897-WB-1_18121409_541.jpg

Cat No. GTX86897

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

18 kDa. ( Note )

Specificity/Sensitivity

Caspase 1 (p20,Cleaved-Asn120) Antibody detects endogenous levels of fragment of activated Caspase 1 resulting from cleavage adjacent to Asn120.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 1.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 1,Ice,Il1Bc,P45,Casp1

Background

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

Database

Research Area

DATA IMAGES
CASP1-cleaved-Asn120-antibody-GTX86897-WB-1_18121409_541.jpg

GTX86897 WB Image

Western blot analysis of extracts from rat eye cells, using Caspase 1 (p20,Cleaved-Asn120) Antibody. The lane on the right is treated with the synthesized peptide.