APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500~1:1000 |
Not tested in other applications.
Calculated MW
Product Note
Caspase 1 (cleaved Asn120) antibody detects endogenous levels of fragment of activated Caspase 1 resulting from cleavage adjacent to Asn120.
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human Caspase 1 (101-150).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CASP1 , ICE , IL1BC , P45 , caspase 1 , Caspase 1
Cellular Localization
Cytoplasm
Background
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
Database
Research Area
DATA IMAGES
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GTX86897 WB Image
WB analysis of rat eye cell lysates using GTX86897 Caspase 1 (cleaved Asn120) antibody. The lane on the right is blocked with the synthesized peptide.
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REFERENCE
There are currently no references for Caspase 1 (cleaved Asn120) antibody (GTX86897). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Caspase 1 (cleaved Asn120) antibody (GTX86897). Be the first to share your experience with this product.