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Caspase 1 (cleaved Asp297) antibody

Anti-Caspase 1 (cleaved Asp297) antibody used in IHC (Paraffin sections) (IHC-P). GTX03738
Anti-Caspase 1 (cleaved Asp297) antibody used in IHC (Paraffin sections) (IHC-P). GTX03738
Anti-Caspase 1 (cleaved Asp297) antibody used in Western Blot (WB). GTX03738

Cat. No. GTX03738

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($429)
See all Caspase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Product Note

This antibody detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Asp297.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human CASP1(Accession P29466), corresponding to amino acid residues I280-V299.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 1 , ICE , IL1BC , P45

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-Caspase 1 (cleaved Asp297) antibody used in IHC (Paraffin sections) (IHC-P). GTX03738

GTX03738 IHC-P Image

IHC-P analysis of rat skin tissue using GTX03738 Caspase 1 (cleaved Asp297) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Caspase 1 (cleaved Asp297) antibody used in IHC (Paraffin sections) (IHC-P). GTX03738

GTX03738 IHC-P Image

IHC-P analysis of rat heart tissue using GTX03738 Caspase 1 (cleaved Asp297) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Caspase 1 (cleaved Asp297) antibody used in Western Blot (WB). GTX03738

GTX03738 WB Image

WB analysis of Jurkat cell lysates using GTX03738 Caspase 1 (cleaved Asp297) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 429