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Caspase 1 p10 subunit antibody

Orthogonal
Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX123675
Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX123675
Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX123675

Cat. No. GTX123675

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Caspase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

pro-form: 45; cleaved form: 10 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Caspase 1 p10 subunit. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168799

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 1 , ICE , IL1BC , P45

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX123675

GTX123675 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Caspase 1 p10 subunit(GTX123675) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX123675

GTX123675 IHC-P Image

Caspase 1 p10 subunit antibody detects Caspase 1 p10 subunit protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Caspase 1 p10 subunit antibody (GTX123675) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 1 p10 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX123675

GTX123675 IHC-P Image

Orthogonal

The data was published in the journal Nutrients in 2018. PMID: 29843457

REFERENCE

Application Reference

Donovan C et al. Immunol Cell Biol 2022; 100 (4) : 235-249 Aim2 suppresses cigarette smoke-induced neutrophil recruitment, neutrophil caspase-1 activation and anti-Ly6G-mediated neutrophil depletion.
Stacchiotti A et al. Nutrients 2018; 10 (6) Taurine Supplementation Alleviates Puromycin Aminonucleoside Damage by Modulating Endoplasmic Reticulum Stress and Mitochondrial-Related Apoptosis in Rat Kidney.
Application : IHC-P
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169