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Caspase 1 p20 subunit antibody

Anti-Caspase 1 p20 subunit antibody used in Western Blot (WB). GTX11701

Cat. No. GTX11701

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse, Rat
Package
100 μg ($439)
See all Caspase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CASP1(137-157aa LEKAQKLWKENPSEIYPIMNT), different from the related rat sequence by three amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2750948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 1 , ICE , Il1bc

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Caspase 1 p20 subunit antibody used in Western Blot (WB). GTX11701

GTX11701 WB Image

WB analysis of various samples using GTX11701 Caspase 1 p20 subunit antibody.
Lane 1 : rat brain tissue lysate
Lane 2 : rat spleen tissue lysate
Lane 3 : mouse brain tissue lysate
Lane 4 : mouse spleen tissue lysate
Lane 5 : mouse testis tissue lysate

REFERENCE

Application Reference

Abd-Ellatif RN et al. Int J Mol Sci 2022; 23 (23) Adrenomedullin Mitigates Doxorubicin-Induced Nephrotoxicity in Rats: Role of Oxidative Stress, Inflammation, Apoptosis, and Pyroptosis.
Yu-Chih Wu et al. Front Med (Lausanne) 2022; 9 : 832713 PEP-sNASP Peptide Alleviates LPS-Induced Acute Lung Injury Through the TLR4/TRAF6 Axis.
Application : WB
El-Sisi AEE et al. Toxicol Appl Pharmacol 2021; 410 : 115340 Octreotide and melatonin alleviate inflammasome-induced pyroptosis through inhibition of TLR4-NF-κB-NLRP3 pathway in hepatic ischemia/reperfusion injury.
Application : WB
Reactivity : Rat
Zhang D et al. J Neurosci Res 2019; (Epub) Gasdermin D serves as a key executioner of pyroptosis in experimental cerebral ischemia and reperfusion model both in vivo and in vitro.
Application : WB
Reactivity : Rat
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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 439