APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes full-length human caspase-10 (59kDa). Since the sequences at C- termini of FLICE2 and Mch4 are different, this antibody recognizes only the FLICE2 form of caspase-10.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide corresponding to aa 505-521 at C-terminus of human FLICE2 (accession no. AAD28402).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ALPS2 , CASP10 , FLICE2 , MCH4 , caspase 10 , Caspase 10
Background
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]
Database
Research Area
DATA IMAGES
|
GTX22012 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22012 Caspase 10 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
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GTX22012 WB Image
Western blot analysis of Caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody (GTX22012) at 1μg/ml.
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REFERENCE
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REVIEW
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