GeneTex
United States (US)

Caspase 12 antibody

Caspase-12-antibody__GTX28117-1_18121409_947.gif
Caspase-12-antibody_WB_GTX28117-1_18121409_101.jpg

Cat No. GTX28117

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

48 kDa. ( Note )

Positive Control

Mouse brain tissue.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide - amino acids 100-116 of Mouse Caspase 12

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Casp12, Caspase12, CASP12P1, Q6UXS9, caspase-12, 12364, 608633, 120329, Caspase 12

Background

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is colocalized to the ER with several proteins that are involved in Alzheimer's disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues (1).

Database

Research Area

DATA IMAGES
Caspase-12-antibody__GTX28117-1_18121409_947.gif

GTX28117 IHC-P Image

Staining of formailin fixed paraffin processed mouse liver with anti-Caspase 12 at 2ug/ml (GTX28117)

Caspase-12-antibody_WB_GTX28117-1_18121409_101.jpg

GTX28117 WB Image

Western blot analysis of Caspase-12 in mouse brain tissue lysate in the absence (A) or presence (B) of blocking peptide with Caspase-12 antibody (GTX28117)at 1μg/ml.

Package List Price ($)
$ 369