GeneTex
United States (US)

Caspase-3 Colorimetric Assay Kit

casp3-color_18121409_669.jpg
GTX85558_CaspaseSubstrateProtocol_19012407_597.jpg

Cat No. GTX85558

PRODUCT

Product Description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

‧ Detection method- Spectrophotometer (400 0r 405 nm) or plate reader
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Kit Contents:
Cell Lysis buffer
2X Reaction Buffer
DEVD-pNA (4 mM)
DTT (1 M)
Dilution Buffer

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase-3 Assay, Caspase 3 Activity Assay, Caspase substrate kit, Apoptosis Assay, Caspase-3 Substrate, Caspase-3 Activity Assay, Caspase3 Substrate, Caspase 3 Substrate, Caspase3 Assay, Caspase3 Activity Assay, Caspase 3 Assay
DATA IMAGES
casp3-color_18121409_669.jpg

GTX85558 Image

Analysis of Caspase-3 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-9 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

GTX85558_CaspaseSubstrateProtocol_19012407_597.jpg

GTX85558 Image


REFERENCE
Package List Price ($)
$ 439