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Caspase-3 Colorimetric Assay Kit

Caspase-3 Colorimetric Assay Kit. GTX85558
Caspase-3 Colorimetric Assay Kit. GTX85558

Cat No. GTX85558

Application

Enzyme Assay

Reactivity

Human
Package
100 assay ($459)
PRODUCT

Summary

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

‧ Detection method- Spectrophotometer (400 0r 405 nm) or plate reader
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Content

  Kit components  Package
1. Cell Lysis Buffer 100 ml
2. 2X Reaction Buffer 4 * 2 ml
3. DEVD-pNA (4 mM) 0.5 ml
4. DTT (1 M) 0.4 ml
5. Dilution Buffer 100 ml

 

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Enzyme Assay Assay dependent
Not tested in other applications.

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Caspase-3 Assay , Caspase 3 Activity Assay , Caspase substrate kit , Apoptosis Assay , Caspase-3 Substrate , Caspase-3 Activity Assay , Caspase3 Substrate , Caspase 3 Substrate , Caspase3 Assay , Caspase3 Activity Assay , Caspase 3 Assay

Research Area

DATA IMAGES

Caspase-3 Colorimetric Assay Kit. GTX85558

GTX85558 Image

Analysis of Caspase-3 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-9 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

Caspase-3 Colorimetric Assay Kit. GTX85558

GTX85558 Image

REFERENCE

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