GeneTex
United States (US)

Caspase 3 antibody [31A1067]

Orthogonal
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GTX13585_407_IHC-P_18121410_816.jpg
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GTX13585_948_WB_18121410_877.jpg
GTX13585_947_WB_18121410_462.jpg
GTX13585_405_IHC-P_18121410_886.jpg
GTX13585_406_IHC-P_18121410_881.jpg
GTX13585_404_IHC-P_18121410_862.jpg
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Cat No. GTX13585

Host Mouse
Clonality Monoclonal
Clone Name 31A1067
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, EM, MS
Reactivity Human, Mouse, Rat, Hamster, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 5 μg/ml
ICC/IF Assay dependent
IHC-P 1:10 - 1:500
IHC-Fr 1:10 - 1:500
FACS Assay dependent
EM Assay dependent
MS Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Specificity/Sensitivity

The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CPP32 antibody , CPP32B antibody , SCA-1 antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX13585_952_WB_18121410_612.jpg

GTX13585 WB Image

Orthogonal

WB analysis of Jurkat cell lysate untreated (-) or treated (+) with Etoposide using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 1 μg/ml

GTX13585_407_IHC-P_18121410_816.jpg

GTX13585 IHC-P Image

IHC-P analysis of human thymus tissue using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 5 μg/ml
Antigen retrieval : Heat-induced antigen retrieval

GTX13585_951_WB_18121410_694.jpg

GTX13585 WB Image

WB analysis of human(Lane 1), mouse(Lane 2), and rat heart lysate(Lane 3) using GTX13585 Caspase 3 antibody [31A1067].

GTX13585_953_WB_18121410_826.jpg

GTX13585 WB Image

Orthogonal

WB analysis of Jurkat cell lysate treated with and without 2 uM staurosporine using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 5 μg/ml

GTX13585_948_WB_18121410_877.jpg

GTX13585 WB Image

WB analysis of 2mM staurosporine treated HeLa cell lysate using GTX13585 Caspase 3 antibody [31A1067].

GTX13585_947_WB_18121410_462.jpg

GTX13585 WB Image

WB analysis of (A) RAW264.7 and (B) NIH-3T3 cell lysate using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 32 μg/ml

GTX13585_405_IHC-P_18121410_886.jpg

GTX13585 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 4 μg/ml

GTX13585_406_IHC-P_18121410_881.jpg

GTX13585 IHC-P Image

IHC-P analysis of human colon tissue using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 4 μg/ml

GTX13585_404_IHC-P_18121410_862.jpg

GTX13585 IHC-P Image

IHC-P analysis of human bladder tissue using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 1:50

GTX13585_949_WB_18121410_898.jpg

GTX13585 WB Image

WB analysis of HeLa cell lysate using GTX13585 Caspase 3 antibody [31A1067].

GTX13585_950_WB_18121410_869.jpg

GTX13585 WB Image

WB analysis of human (A) brain, (B) heart, (C) intestine, (D) kidney, (E) liver, (F) lung, (G) muscle, (H) stomach, (I) spleen, (J) ovary, and (K) testis tissue lysate using GTX13585 Caspase 3 antibody [31A1067].