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Caspase 3 antibody [31A1067]

Orthogonal
Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585
Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585
Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585
Anti-Caspase 3 antibody [31A1067] used in IHC (Paraffin sections) (IHC-P). GTX13585
Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585
Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585
Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585
Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585
Anti-Caspase 3 antibody [31A1067] used in IHC (Paraffin sections) (IHC-P). GTX13585
Anti-Caspase 3 antibody [31A1067] used in IHC (Paraffin sections) (IHC-P). GTX13585
Anti-Caspase 3 antibody [31A1067] used in IHC (Paraffin sections) (IHC-P). GTX13585

Cat. No. GTX13585

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 31A1067

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, EM, MS

Reactivity

 Human, Mouse, Rat, Hamster, Pig
Package
100 μg ($469)
See all Caspase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 5 μg/ml
ICC/IF Assay dependent
IHC-P 1:10 - 1:500
IHC-Fr 1:10 - 1:500
FACS Assay dependent
EM Assay dependent
MS Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 3 , CPP32 , CPP32B , SCA-1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585

GTX13585 WB Image

WB analysis of human (A) brain, (B) heart, (C) intestine, (D) kidney, (E) liver, (F) lung, (G) muscle, (H) stomach, (I) spleen, (J) ovary, and (K) testis tissue lysate using GTX13585 Caspase 3 antibody [31A1067].

Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585

GTX13585 WB Image

Orthogonal

WB analysis of Jurkat cell lysate untreated (-) or treated (+) with Etoposide using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 1 μg/ml

Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585

GTX13585 WB Image

WB analysis of (A) RAW264.7 and (B) NIH-3T3 cell lysate using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 32 μg/ml

Anti-Caspase 3 antibody [31A1067] used in IHC (Paraffin sections) (IHC-P). GTX13585

GTX13585 IHC-P Image

IHC-P analysis of human colon tissue using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 4 μg/ml

Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585

GTX13585 WB Image

Orthogonal

WB analysis of Jurkat cell lysate treated with and without 2 uM staurosporine using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 5 μg/ml

Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585

GTX13585 WB Image

WB analysis of human(Lane 1), mouse(Lane 2), and rat heart lysate(Lane 3) using GTX13585 Caspase 3 antibody [31A1067].

Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585

GTX13585 WB Image

WB analysis of HeLa cell lysate using GTX13585 Caspase 3 antibody [31A1067].

Anti-Caspase 3 antibody [31A1067] used in Western Blot (WB). GTX13585

GTX13585 WB Image

WB analysis of 2mM staurosporine treated HeLa cell lysate using GTX13585 Caspase 3 antibody [31A1067].

Anti-Caspase 3 antibody [31A1067] used in IHC (Paraffin sections) (IHC-P). GTX13585

GTX13585 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 4 μg/ml

Anti-Caspase 3 antibody [31A1067] used in IHC (Paraffin sections) (IHC-P). GTX13585

GTX13585 IHC-P Image

IHC-P analysis of human thymus tissue using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 5 μg/ml
Antigen retrieval : Heat-induced antigen retrieval

Anti-Caspase 3 antibody [31A1067] used in IHC (Paraffin sections) (IHC-P). GTX13585

GTX13585 IHC-P Image

IHC-P analysis of human bladder tissue using GTX13585 Caspase 3 antibody [31A1067].
Dilution : 1:50

REFERENCE

Application Reference

Hernandez A et al. PLoS One 2022; 17 (12) : e0278516 Dihydroxyacetone suppresses mTOR nutrient signaling and induces mitochondrial stress in liver cells.
Application : WB
Reactivity : Human
Ma C et al. Evid Based Complement Alternat Med 2020; 2020 : 4675851 Radix Angelica Sinensis and Radix Hedysari Ultrafiltration Extract Protects against X-Irradiation-Induced Cardiac Fibrosis in Rats.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469