Caspase 3 antibody [5E1]

Cat. No. GTX34164

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	5E1
Isotype	IgG1
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($419)

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View product citations for antibody GTX34164 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
IHC-P	1:100-1:200

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887734

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CASP3,CPP32,CPP32B,SCA1,caspase 3,Caspase 3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Gene ID: 836 CASP3
UniProt: P42574 CASP3

Research Area

DATA IMAGES

GTX34164 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34164 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34164 IHC-P Image

IHC-P analysis of human liver tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34164 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34164 Caspase 3 antibody [5E1].

GTX34164 IHC-P Image

IHC-P analysis of rat lung tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34164 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34164 WB Image

WB analysis of various sample lysates using GTX34164 Caspase 3 antibody [5E1].
Lane 1 : HeLa cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : Rat brain tissue lysate

GTX34164 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34164 IHC-P Image

IHC-P analysis of human lung tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

REFERENCE

Application Reference

Chang YH et al. Tzu Chi Medical Journal 2018; (Epub) Human umbilical cord-derived mesenchymal stem cells reduce monosodium iodoacetate-induced apoptosis in cartilage.

Application : IHC-P

Reactivity : Human

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REVIEW

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Application Reference

Chang YH et al. Tzu Chi Medical Journal 2018; (Epub) Human umbilical cord-derived mesenchymal stem cells reduce monosodium iodoacetate-induced apoptosis in cartilage.

Application : IHC-P

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 419

Qty

Caspase 3 antibody [5E1]

Cat. No. GTX34164

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX34164 IHC-P Image

GTX34164 IHC-P Image

GTX34164 IHC-P Image

GTX34164 IHC-P Image

GTX34164 IHC-P Image

GTX34164 IHC-P Image

GTX34164 WB Image

GTX34164 IHC-P Image

GTX34164 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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