GeneTex
United States (US)

Caspase 3 antibody [5E1]

GTX34164_20170112_WB_18121410_450.jpg
GTX34164_20170112_IHC-P_18121410_568.jpg
GTX34164_20170112_WB_1_18121410_198.jpg

Cat No. GTX34164

Host Mouse
Clonality Monoclonal
Clone Name 5E1
Isotype IgG1
Application WB, IHC-P
Reactivity Human, Mouse, Rat, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

32 kDa kDa. ( Note )

Specificity/Sensitivity

The antibody detects endogenous Active Caspase-3 protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CPP32 Antibody , SCA1 Antibody , CASP3 Antibody , CPP32B Antibody , caspase 3 Antibody

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX34164_20170112_WB_18121410_450.jpg

GTX34164 WB Image

Western Blot (WB) analysis of 1) HeLa, 2) 3T3, 3) Rat Brain Tissue using Active Caspase-3 Monoclonal Antibody.

GTX34164_20170112_IHC-P_18121410_568.jpg

GTX34164 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil Tissue using Active Caspase-3 Monoclonal Antibody.

GTX34164_20170112_WB_1_18121410_198.jpg

GTX34164 WB Image

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000.

Reference