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Caspase 3 antibody [5E1]

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in Western Blot (WB). GTX34164

Cat No. GTX34164

Host

Mouse

Clonality

Monoclonal

Clone Name

5E1

Isotype

IgG1

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:100-1:200
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CASP3,CPP32,CPP32B,SCA1,caspase 3,Caspase 3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

IHC-P analysis of human liver tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

IHC-P analysis of human lung tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

IHC-P analysis of rat lung tissue using GTX34164 Caspase 3 antibody [5E1]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34164 Caspase 3 antibody [5E1].

Anti-Caspase 3 antibody [5E1] used in Western Blot (WB). GTX34164

GTX34164 WB Image

WB analysis of various sample lysates using GTX34164 Caspase 3 antibody [5E1].
Lane 1 : HeLa cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : Rat brain tissue lysate

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319