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Caspase 3 antibody [5E1]

Anti-Caspase 3 antibody [5E1] used in Western Blot (WB). GTX34164
Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164
Anti-Caspase 3 antibody [5E1] used in Western Blot (WB). GTX34164

Cat No. GTX34164

Host

Mouse

Clonality

Monoclonal

Clone Name

5E1

Isotype

IgG1

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Chicken
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Specificity/Sensitivity

The antibody detects endogenous Active Caspase-3 protein.

PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Caspase 3 , Cpp32 , Cpp32B , Sca-1 , Casp3

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 antibody [5E1] used in Western Blot (WB). GTX34164

GTX34164 WB Image

Western Blot (WB) analysis of 1) HeLa, 2) 3T3, 3) Rat Brain Tissue using Active Caspase-3 Monoclonal Antibody.

Anti-Caspase 3 antibody [5E1] used in IHC (Paraffin sections) (IHC-P). GTX34164

GTX34164 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil Tissue using Active Caspase-3 Monoclonal Antibody.

Anti-Caspase 3 antibody [5E1] used in Western Blot (WB). GTX34164

GTX34164 WB Image

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000.

REFERENCE

REVIEW

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Package List Price ($)
$ 319