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Caspase 3 antibody [5H10]

Anti-Caspase 3 antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX34166
Anti-Caspase 3 antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX34166
Anti-Caspase 3 antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX34166

Cat No. GTX34166

Host Mouse
Clonality Monoclonal
Clone Name 5H10
Isotype IgG1
Application IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Specificity/Sensitivity

Caspase-3 Mouse Monoclonal Antibody (5H10) detects endogenous levels of CASP3
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic Peptide of Caspase-3

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 3,Cpp32,Cpp32B,Sca-1,Casp3

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Caspase 3 antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX34166

GTX34166 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Prostate Tissue using Caspase-3 Mouse Monoclonal Antibody diluted at 1:200.

Anti-Caspase 3 antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX34166

GTX34166 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skin Tissue using Caspase-3 Mouse Monoclonal Antibody diluted at 1:200.

Anti-Caspase 3 antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX34166

GTX34166 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Liver Tissue using Caspase-3 Mouse Monoclonal Antibody diluted at 1:200.