GeneTex
United States (US)

Caspase 3 antibody [CPP32 4-1-18]

GTX30246_543_IHC-P_18121410_932.jpg
GTX30246_1379_WB_18121410_245.jpg
GTX30246_544_IHC-P_18121410_573.jpg
GTX30246_545_IHC-P_18121410_191.jpg
GTX30246_1380_WB_18121410_862.jpg

Cat No. GTX30246

Host Mouse
Clonality Monoclonal
Clone Name CPP32 4-1-18
Isotype IgG2a
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:200 - 1:500
Not tested in other applications.

Calculated MW

32 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant human Caspase 3 [UniProt# P42574]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CPP32 antibody , CPP32B antibody , SCA-1 antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30246_543_IHC-P_18121410_932.jpg

GTX30246 IHC-P Image

IHC-P analysis of human bladder tissue using GTX30246 Caspase 3 antibody [CPP32 4-1-18].
Dilution : 1:300

GTX30246_1379_WB_18121410_245.jpg

GTX30246 WB Image

WB analysis of HEK293 cell lysate using GTX30246 Caspase 3 antibody [CPP32 4-1-18].

GTX30246_544_IHC-P_18121410_573.jpg

GTX30246 IHC-P Image

IHC-P analysis of human spleen tissue using GTX30246 Caspase 3 antibody [CPP32 4-1-18].
Dilution : 1:200

GTX30246_545_IHC-P_18121410_191.jpg

GTX30246 IHC-P Image

IHC-P analysis of rat tongue base tissue using GTX30246 Caspase 3 antibody [CPP32 4-1-18].
Antigen retrieval : Citrate buffer pH 6

GTX30246_1380_WB_18121410_862.jpg

GTX30246 WB Image

WB analysis of HEK293 cell lysate using GTX30246 Caspase 3 antibody [CPP32 4-1-18].
Lanes 1 : inactive Caspase
Lane 2 : active Caspase