APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rabbit, Bovine, Cat, Pig, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910078
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase 3 , CPP32 , CPP32B , SCA-1
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/templates/default/js/lightbox/images/loading.gif "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
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GTX636810 WB Image
Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636810 WB Image
Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636810 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 (cleaved Asp175) antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636810 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636810 IHC-P Image
Caspase 3 antibody [HL1370] detects Caspase 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Caspase 3 stained by Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for Caspase 3 antibody [HL1370] (GTX636810). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Caspase 3 antibody [HL1370] (GTX636810). Be the first to share your experience with this product.
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_44662_20220513_WB_KO_watermark_w_23061202_600.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_T-44599_20220408_WB_R_treatment_Cisplatin_w_23061202_716.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_T-44599_20220318_WB_R_w_23061202_829.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_44662_20220429_WB_treatment_Cisplatin_w_23061202_958.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in IHC (Paraffin sections) (IHC-P). GTX636810](/upload/website/prouct_img/small/GTX636810/GTX636810_44662_20220527_IHC-P_22062121_242.webp "Anti-Caspase 3 antibody [HL1370] used in IHC (Paraffin sections) (IHC-P). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_44662_20220513_WB_KO_watermark_w_23061202_600.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_T-44599_20220408_WB_R_treatment_Cisplatin_w_23061202_716.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_T-44599_20220318_WB_R_w_23061202_829.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_44662_20220429_WB_treatment_Cisplatin_w_23061202_958.webp "Anti-Caspase 3 antibody [HL1370] used in Western Blot (WB). GTX636810")
![Anti-Caspase 3 antibody [HL1370] used in IHC (Paraffin sections) (IHC-P). GTX636810](/upload/website/prouct_img/normal/GTX636810/GTX636810_44662_20220527_IHC-P_22062121_242.webp "Anti-Caspase 3 antibody [HL1370] used in IHC (Paraffin sections) (IHC-P). GTX636810")