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Caspase 3 antibody

Anti-Caspase 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00752

Cat No. GTX00752

Host

Rabbit

Clonality

Polyclonal

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:3000
ICC/IF 1:500-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes the active form of human caspase 3, p20/p17 subunit but does not recognize the proenzyme p32.

PROPERTIES

Form

Liquid

Buffer

Serum with 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the caspase 3 cleavage site, 6 aa (CGIETD)

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 3 , CPP32 , CPP32B , SCA-1

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00752

GTX00752 ICC/IF Image

ICC/IF analysis of NT2 neurons using GTX00752 Caspase 3 antibody. Triply stained for the N-terminus of APP (with P2-1) or beta-gal (with anti- beta gal antibody), chromosomal DNA (Hoechst), and activated caspase 3 subunits (with GTX00752). Some neurons accumulating APP are strongly immunostained with GTX00752 (arrows), whereas neurons accumulating beta-gal are hardly labeled (arrowheads).
Fixation : 4% PFA

REFERENCE

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REVIEW

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Package List Price ($)
$ 359