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Caspase 3 antibody

KO/KD Orthogonal
Anti-Caspase 3 antibody used in Western Blot (WB). GTX123678
Anti-Caspase 3 antibody used in Western Blot (WB). GTX123678
Anti-Caspase 3 antibody used in Western Blot (WB). GTX123678
Anti-Caspase 3 antibody used in Western Blot (WB). GTX123678

Cat. No. GTX123678

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Plutella xylostella
Package
100 μl ($399),
25 μl ($169)
See all Caspase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:35918763)

Predict Reactivity

Rabbit, Bovine, Cat, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176802

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 3 , CPP32 , CPP32B , SCA-1

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 antibody used in Western Blot (WB). GTX123678

GTX123678 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A antibody (GTX123678) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in Western Blot (WB). GTX123678

GTX123678 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX123678) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in Western Blot (WB). GTX123678

GTX123678 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX123678) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 3 antibody used in Western Blot (WB). GTX123678

GTX123678 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX123678) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hung MC et al. Cell Biosci 2022; 12 (1) : 122 AKT phosphorylation as a predictive biomarker for PI3K/mTOR dual inhibition-induced proteolytic cleavage of mTOR companion proteins in small cell lung cancer.
Application : WB
Reactivity : Human
Chen WJ et al. PLoS Pathog 2014; 10 (8) : e1004288 Characterization of an Insecticidal Toxin and Pathogenicity of Pseudomonas taiwanensis against Insects.
Application : IHC-P
Reactivity : Plutella xylostella
Submit a Reference

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Package List Price ($)
$ 399
$ 169