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Caspase 3 (cleaved Asp175) antibody

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX03281
Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX03281
Anti-Caspase 3 (cleaved Asp175) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03281
Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX03281
Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX03281

Cat. No. GTX03281

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all Caspase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from Human CASP3 (Accession P42574), corresponding to amino acid residues C163-M182.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CASP3,CPP32,CPP32B,SCA1,caspase 3,Caspase 3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX03281

GTX03281 IHC-P Image

IHC-P analysis of human ehrlich carcimoma tissue using GTX03281 Caspase 3 (cleaved Asp175) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX03281

GTX03281 WB Image

WB analysis of various sample lysates using GTX03281 Caspase 3 (cleaved Asp175) antibody.
Lane 1 : HeLa treated with blocking peptide
Lane 2 : HeLa (etoposide treated, 25μM 5h)
Lane 3 : MCF7 (etoposide treated, 25μM 5h)
Lane 4 : Mouse heart tissue lysate
Lane 5 : Mouse spleen tissue lysate

Anti-Caspase 3 (cleaved Asp175) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03281

GTX03281 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX03281 Caspase 3 (cleaved Asp175) antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX03281

GTX03281 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX03281 Caspase 3 (cleaved Asp175) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX03281

GTX03281 WB Image

WB analysis of 293T lysate using GTX03281 Caspase 3 (cleaved Asp175) antibody. The lane on the left was treated with blocking peptide.

REFERENCE

Application Reference

Li Y et al. Exp Ther Med 2022; 24 (3) : 567 Interleukin 32 participates in cardiomyocyte-induced oxidative stress, inflammation and apoptosis during hypoxia/reoxygenation via the NOD2/NOX2/MAPK signaling pathway.
Application : WB
Reactivity : Human
Submit a Reference

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Package List Price ($)
$ 419