WB analysis of 293T lysate using GTX03281 Caspase 3 (cleaved Asp175) antibody. The lane on the left was treated with blocking peptide.

IHC-P analysis of mouse brain tissue using GTX03281 Caspase 3 (cleaved Asp175) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

ICC/IF analysis of PFA-fixed HeLa cells using GTX03281 Caspase 3 (cleaved Asp175) antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

WB analysis of various sample lysates using GTX03281 Caspase 3 (cleaved Asp175) antibody.
Lane 1 : HeLa treated with blocking peptide
Lane 2 : HeLa (etoposide treated, 25μM 5h)
Lane 3 : MCF7 (etoposide treated, 25μM 5h)
Lane 4 : Mouse heart tissue lysate
Lane 5 : Mouse spleen tissue lysate

IHC-P analysis of human ehrlich carcimoma tissue using GTX03281 Caspase 3 (cleaved Asp175) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100