GeneTex
United States (US)

Caspase 3 (cleaved Asp175) antibody

Caspase-3-cleaved-Asp175-antibody__GTX86900-1_18121409_826.jpg
Caspase-3-cleaved-Asp175-antibody__GTX86900-2_18121409_953.jpg

Cat No. GTX86900

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

Recommended Starting Dilutions: For WB: Use at a dilution of 1:500-1:1000. For IHC-P: Use at a dilution of 1:50-1:100. For ELISA: Use at a dilution of 1:20000. Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

32 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 157 and 206 of human Caspase 3.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 3,Cpp32,Cpp32B,Sca-1,Casp3

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Database

Research Area

DATA IMAGES
Caspase-3-cleaved-Asp175-antibody__GTX86900-1_18121409_826.jpg

GTX86900 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Caspase 3 (Cleaved-Asp175) Antibody. The picture on the right is treated with the synthesized peptide.

Caspase-3-cleaved-Asp175-antibody__GTX86900-2_18121409_953.jpg

GTX86900 WB Image

Western blot analysis of extracts from HeLa cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) antibody (GTX86900). The lane on the right is treated with the synthesized peptide.