GeneTex
United States (US)

Caspase 3 (cleaved Asp175) antibody

Caspase-3-cleaved-Asp175-antibody-GTX86909-IHC-1_18121409_542.jpg

Cat No. GTX86909

Host Rabbit
Clonality Polyclonal
Application IHC-P, ELISA, IHC
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

20 and 35 kDa. ( Note )

Specificity/Sensitivity

Caspase 3 (Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 3.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 3,Cpp32,Cpp32B,Sca-1,Casp3

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Database

Research Area

DATA IMAGES
Caspase-3-cleaved-Asp175-antibody-GTX86909-IHC-1_18121409_542.jpg

GTX86909 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Caspase 3 (Cleaved-Asp175) Antibody. The picture on the right is treated with the synthesized peptide.

REFERENCE