GeneTex
United States (US)

Caspase 3 (cleaved Asp175) antibody

GTX86952_WB_18121411_302.jpg
Caspase-3-cleaved-Asp175-antibody__GTX86952-1_18121409_886.jpg

Cat No. GTX86952

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
ELISA 1:20000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The immunogen was produced against synthesized peptide(126-175a.a) derived from human Caspase 3

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 3,Cpp32,Cpp32B,Sca-1,Casp3

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Database

Research Area

DATA IMAGES
GTX86952_WB_18121411_302.jpg

GTX86952 WB Image

Western blot analysis of extracts from 293 cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) Antibody.(GTX86952) The lane on the right is treated with the synthesized peptide.

Caspase-3-cleaved-Asp175-antibody__GTX86952-1_18121409_886.jpg

GTX86952 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 3 (Cleaved-Asp175) antibody (GTX86952). The picture on the right is treated with the synthesized peptide.

REFERENCE
Application Reference