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Caspase 3 (cleaved Asp175) antibody

Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX86952
Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX86952

Cat No. GTX86952

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
ELISA 1:20000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen was produced against synthesized peptide(126-175a.a) derived from human Caspase 3

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Caspase 3 , Cpp32 , Cpp32B , Sca-1 , Casp3

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Database

Research Area

DATA IMAGES

Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX86952

GTX86952 WB Image

Western blot analysis of extracts from 293 cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) Antibody.(GTX86952) The lane on the right is treated with the synthesized peptide.

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX86952

GTX86952 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 3 (Cleaved-Asp175) antibody (GTX86952). The picture on the right is treated with the synthesized peptide.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329