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Caspase 3 (cleaved Asp175) antibody

Orthogonal
Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX86952
Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX86952
Anti-Caspase 3 (cleaved Asp175) antibody used in Immunohistochemistry (IHC). GTX86952

Cat. No. GTX86952

Reference 30

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Caspase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
IHC Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 3.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10722506

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CASP3,CPP32,CPP32B,SCA1,caspase 3,Caspase 3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 (cleaved Asp175) antibody used in Western Blot (WB). GTX86952

GTX86952 WB Image

WB analysis of 293 cells treated with Etoposide (25uM, 60mins) lysate using GTX86952 Caspase 3 (cleaved Asp175) antibody.

Anti-Caspase 3 (cleaved Asp175) antibody used in IHC (Paraffin sections) (IHC-P). GTX86952

GTX86952 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX86952 Caspase 3 (cleaved Asp175) antibody.

Anti-Caspase 3 (cleaved Asp175) antibody used in Immunohistochemistry (IHC). GTX86952

GTX86952 IHC Image

Orthogonal

The data was published in the journal Sci Rep in 2019. PMID: 31366973

REFERENCE

Application Reference

Sun Y et al. Korean J Physiol Pharmacol 2024; 28 (1) : 1-10 Carvacrol improves blood lipid and glucose in rats with type 2 diabetes mellitus by regulating short-chain fatty acids and the GPR41/43 pathway.
Y Ke et al. Research Square 2024; Glioma stem cells deliver ABCB4 transcribed by ATF3 via exosomes conferring glioma resistance to temozolomide
Sun Z et al. Front Cell Neurosci 2022; 16 : 911973 Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation.
Yuan-Kun Tu et al. Curr Neurovasc Res 2021; 18 (4) : 395-408 Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
Application : WB
Reactivity : Human
Xu C et al. Oxid Med Cell Longev 2021; 2021 : 2502324 Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm.
Application : WB, IHC-P
Reactivity : Mouse
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REVIEW

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Application Reference
Sun Y et al. Korean J Physiol Pharmacol 2024; 28 (1):1-10 Carvacrol improves blood lipid and glucose in rats with type 2 diabetes mellitus by regulating short-chain fatty acids and the GPR41/43 pathway.
Y Ke et al. Research Square 2024; Glioma stem cells deliver ABCB4 transcribed by ATF3 via exosomes conferring glioma resistance to temozolomide
Sun Z et al. Front Cell Neurosci 2022; 16:911973 Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation.
Yuan-Kun Tu et al. Curr Neurovasc Res 2021; 18 (4):395-408 Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
Application : WB
Reactivity : Human
Xu C et al. Oxid Med Cell Longev 2021; 2021:2502324 Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm.
Application : WB, IHC-P
Reactivity : Mouse
Du D. et al. Journal of Functional Foods 2021; 84:104584 Dietary supplementation of camel whey protein attenuates heat stress-induced liver injury by inhibiting NLRP3 inflammasome activation through the HMGB1/RAGE signalling pathway.
Application : IHC-P
Zhang J et al. Mediators Inflamm 2021; 2021:6611085 Anti-Interleukin-16-Neutralizing Antibody Attenuates Cardiac Inflammation and Protects against Cardiac Injury in Doxorubicin-Treated Mice.
Application : WB
Reactivity : Mouse
Li D et al. Life Sci 2021; 277:119467 Maresin 1 alleviates the inflammatory response, reduces oxidative stress and protects against cardiac injury in LPS-induced mice.
Application : WB
Reactivity : Mouse
Huang C et al. BMC Complement Med Ther 2020; 20 (1):68 Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and -independent apoptosis via Smad or Akt signaling pathways in HOS cells.
Yan J et al. Life Sci 2020; 263:118586 Carbon ion combined with tigecycline inhibits lung cancer cell proliferation by inducing mitochondrial dysfunction.
Application : WB
Reactivity : Human
Li M et al. Cancer Manag Res 2020; 12:9159-9171
Application : WB
Reactivity : Human
Ahn CR et al. Biomedicines 2020; 8 (9) Cinnamaldehyde and Hyperthermia Co-Treatment Synergistically Induces ROS-Mediated Apoptosis in ACHN Renal Cell Carcinoma Cells.
Application : WB
Reactivity : Human
Xu Y et al. Aging (Albany NY) 2020; 12 (14):14205-14218 Lowered levels of nicotinic acetylcholine receptors and elevated apoptosis in the hippocampus of brains from patients with type 2 diabetes mellitus and db/db mice.
Application : WB
Reactivity : Mouse
Wang YY et al. ACS Omega 2020; 5 (12):7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
Application : WB
Reactivity : Human
Tan YJ et al. Ther Adv Med Oncol 2019; 11:1758835919878977 BZD9L1 sirtuin inhibitor as a potential adjuvant for sensitization of colorectal cancer cells to 5-fluorouracil.
Application : WB
Reactivity : Human
Yi Jer Tan et al. Therapeutic Advances in Medical Oncology 2019; (Epub) BZD9L1 sirtuin inhibitor as a potential adjuvant for sensitization of colorectal cancer cells to 5-fluorouracil.
Application : WB
Reactivity : Human
Chang CS et al. Sci Rep 2019; 9 (1):11108 Enterovirus 71 targets the cardiopulmonary system in a robust oral infection mouse model.
Application : IHC-P
Reactivity : Mouse
Dasgupta A et al. IUBMB Life 2019; (Epub) Astrakurkurone, a Sesquiterpenoid from Wild Edible Mushroom, Targets Liver Cancer Cells by Modulating Bcl-2 Family Proteins.
Application : WB
Reactivity : Human
Si W et al. 3 Biotech 2019; 9 (4):131 Erythropoietin protects neurons from apoptosis via activating PI3K/AKT and inhibiting Erk1/2 signaling pathway.
Application : WB
Reactivity : Mouse
Yu H et al. Microcirculation 2019;:e12537 Effect of mild hypothermia on cerebral microcirculation in a murine cardiopulmonary resuscitation model.
Application : WB
Reactivity : Rat
Chen CC et al. Cancers (Basel) 2018; 10 (9) Melatonin Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy Through Long Non-Coding RNA RAD51-AS1-Mediated Suppression of DNA Repair.
Application : WB
Reactivity : Human
Chen CC et al. Cell Death Dis 2018; 9 (5):543 Corylin increases the sensitivity of hepatocellular carcinoma cells to chemotherapy through long noncoding RNA RAD51-AS1-mediated inhibition of DNA repair.
Application : WB
Reactivity : Human
Yan J et al. J Photochem Photobiol B 2018; MiR-340/iASPP axis affects UVB-mediated retinal pigment epithelium (RPE) cell damage.
Application : WB
Reactivity : Human
Omer FAA et al. Tumour Biol 2017; 39 (11):1.01E+15 Beta-mangostin from Cratoxylum arborescens activates the intrinsic apoptosis pathway through reactive oxygen species with downregulation of the HSP70 gene in the HL60 cells associated with a G0/G1 cell-cycle arrest.
Application : WB
Reactivity : Human
He Y et al. FASEB J 2017; (Epub) Adeno-associated viral 9-mediated Cdk5 inhibitory peptide reverses pathologic changes and behavioral deficits in the Alzheimer's disease mouse model.
Application : WB
Reactivity : Human
Huang ST et al. Sci Rep 2017; 7:40382 Tanshinone IIA induces intrinsic apoptosis in osteosarcoma cells both in vivo and in vitro associated with mitochondrial dysfunction.
Application : IHC-P
Reactivity : Human
Zheng Y et al. Int J Biol Sci 2016; 12 (1):120-32 Photodynamic-therapy Activates Immune Response by disrupting Immunity Homeostasis of Tumor Cells, which Generates Vaccine for Cancer Therapy.
Application : WB
Reactivity : Mouse
Huang ST et al. Am J Chin Med 2016; 44 (7):1507-1523 Phyllanthus urinaria's Inhibition of Human Osteosarcoma Xenografts Growth in Mice is Associated with Modulation of Mitochondrial Fission/Fusion Machinery.
Application : IHC-P
Reactivity : Mouse
Qu J et al. Clin Exp Pharmacol Physiol 2015; (Epub) PFKFB3 modulates glycolytic metabolism and alleviates endoplasmic reticulum stress in human osteoarthritis cartilage.
Application : WB
Reactivity : Human
Dong Y et al. J Nat Prod 2012; 75 (3):370-7 Antitumor agents. 289. Design, synthesis, and anti-breast cancer activity in vivo of 4-amino-2H-benzo[h]chromen-2-one and 4-amino-7,8,9,10-tetrahydro-2H-benzo[h]chromen-2-one analogues with improved water solubility.
Package List Price ($)
$ 399