GeneTex
  • Country / Location Selection

United States (US)

Caspase 3 p17 subunit antibody

Anti-Caspase 3 p17 subunit antibody used in Western Blot (WB). GTX49360
Anti-Caspase 3 p17 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX49360

Cat No. GTX49360

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($349)

APPLICATION

Application Note

WB: 1:500 - 1:1000, IHC-P: 1:50 - 1:200

Calculated MW

32 kDa. ( Note )

Specificity/Sensitivity

Actived-Caspase-3 p17 pAb detects endogenous levels of Actived-Caspase-3 p17 protein.

PROPERTIES

Form

Liquid

Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 150 - 200 of Human active Caspase 3.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Caspase 3 , Cpp32 , Cpp32B , Sca-1 , Casp3

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Caspase 3 p17 subunit antibody used in Western Blot (WB). GTX49360

GTX49360 WB Image

Western blot (WB) analysis of Actived-Caspase-3 p17 pAb at 1:500 dilution. Lane1:Hela whole cell lysate(40ug). Lane2:Hela treated with H2O2 (100nmol/ml, 25min, phostop) whole cell lysate (40ug). Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase)

Anti-Caspase 3 p17 subunit antibody used in IHC (Paraffin sections) (IHC-P). GTX49360

GTX49360 IHC-P Image

Immunohistochemistry (IHC) analyzes of Actived-Caspase-3 p17 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

REFERENCE

There are currently no references for Caspase 3 p17 subunit antibody (GTX49360). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Caspase 3 p17 subunit antibody (GTX49360). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf