0.01M PBS pH7.4, 1% BSA, 15 mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
synthetic peptide corresponding to human procaspase 4α isoform sequence, (amino acids 81-98) with N-terminal lysine added, conjugated to KLH.
Purified by affinity chromatography
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Caspase 4 is a 377 amino acids protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an upstream caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67% homology to mature caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.