Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX113639) diluted at 1:1000.

Caspase 4 antibody detects Caspase 4 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Caspase 4 antibody (GTX113639) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX113639) diluted at 1:1000.

Caspase 4 antibody detects CASP4 protein at cytosol on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
Caspase 4 antibody (GTX113639) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113639 diluted at 1:1000

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX113639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal Microorganisms in 2019.PMID: 31096680