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Caspase 4 antibody

Overexpression
Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639
Anti-Caspase 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113639
Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639
Anti-Caspase 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113639
Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639
Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639
Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639

Cat. No. GTX113639

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Dog, Pig
Package
100 μl ($399),
25 μl ($169)
See all Caspase 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

H1299 , myc-tagged human caspas4-transfected 293T cells , K562 , THP-1 , HL-60

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036446

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 4 , ICE(rel)II , ICEREL-II , ICH-2 , Mih1 , Mih1/TX , TX

Background

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639

GTX113639 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX113639) diluted at 1:1000.

Anti-Caspase 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113639

GTX113639 IHC-P Image

Caspase 4 antibody detects CASP4 protein at cytosol on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
Caspase 4 antibody (GTX113639) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639

GTX113639 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX113639) diluted at 1:1000.

Anti-Caspase 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113639

GTX113639 IHC-P Image

Caspase 4 antibody detects Caspase 4 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Caspase 4 antibody (GTX113639) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639

GTX113639 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX113639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639

GTX113639 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113639 diluted at 1:1000

Anti-Caspase 4 antibody used in Western Blot (WB). GTX113639

GTX113639 WB Image

The data was published in the journal Microorganisms in 2019.PMID: 31096680

REFERENCE

Application Reference

Liu X et al. Microorganisms 2019; 7 (5) Oral Administration of a Select Mixture of Lactobacillus and Bacillus Alleviates Inflammation and Maintains Mucosal Barrier Integrity in the Ileum of Pigs Challenged with Salmonella Infantis.
Application : WB
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169