Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX134552) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX134552) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

THP-1 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 4 (cleaved Gln81) antibody (GTX134552) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Caspase 4 antibody detects Caspase 4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Caspase 4 stained by Caspase 4 antibody (GTX134552) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Caspase 4 antibody detects Caspase 4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Caspase 4 stained by Caspase 4 antibody (GTX134552) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX134552) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.