GeneTex
United States (US)

Caspase 5 antibody

GTX31700_2866_WB_20180221_18121410_947.jpg
GTX31700_2865_WB_20180221_18121410_879.jpg

Cat No. GTX31700

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human
See all Caspase 5 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Ramos Cell Lysate(GTX23955) , Human Spleen Tissue Lysate(GTX27921) , Human Thymus Tissue Lysate

Specificity/Sensitivity

Depending on cell lines or tissues used, other cleavage products may be observed.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Caspase-5 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-5.The immunogen is located within amino acids 40 - 90 of Caspase-5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 5,Ice(Rel)Iii,Icerel-Iii,Ich-3,Casp5

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES
GTX31700_2866_WB_20180221_18121410_947.jpg

GTX31700 WB Image

WB analysis of Ramos cell lysate using GTX31700 Caspase 5 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml
GTX31700_2865_WB_20180221_18121410_879.jpg

GTX31700 WB Image

WB analysis of (A) human spleen tissue and (B) human thymus lysate using GTX31700 Caspase 5 antibody.
Working concentration : 1 μg/ml
Package List Price ($)
$ 339