APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 2 μg/mL |
| 2 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Ramos Cell Lysate(GTX23955) , P815 Cell Lysate(GTX27185) , P815 Cell Slide(GTX25547)
Product Note
Depending on cell lines or tissues used, other cleavage products may be observed.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Caspase-5 antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5.The immunogen is located within amino acids 170 - 220 of Caspase-5.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase 5 , ICE(rel)III , ICEREL-III , ICH-3
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
Database
Research Area
DATA IMAGES
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GTX31701 ICC/IF Image
ICC/IF analysis of P815 cells using GTX31701 Caspase 5 antibody.
Working concentration : 2 μg/ml
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GTX31701 WB Image
WB analysis of Ramos cell lysate using GTX31701 Caspase 5 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml
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GTX31701 ICC/IF Image
ICC/IF analysis of P815 cells using GTX31701 Caspase 5 antibody.
Working concentration : 2 μg/ml
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REFERENCE
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REVIEW
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