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Caspase 6 antibody [GT1154]

Recombinant antibody Orthogonal
Anti-Caspase 6 antibody [GT1154] used in Western Blot (WB). GTX01097
Anti-Caspase 6 antibody [GT1154] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01097
Anti-Caspase 6 antibody [GT1154] used in Western Blot (WB). GTX01097
Anti-Caspase 6 antibody [GT1154] used in Western Blot (WB). GTX01097

Cat. No. GTX01097

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1154

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399)
See all Caspase 6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Caspase-6 p18.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 6 , MCH2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Caspase 6 antibody [GT1154] used in Western Blot (WB). GTX01097

GTX01097 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 6 antibody [GT1154] (GTX01097) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 20682790

Anti-Caspase 6 antibody [GT1154] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01097

GTX01097 ICC/IF Image

ICC/IF analysis of C6 cells using GTX01097 Caspase 6 antibody [GT1154].
Orange: Primary antibody
Blue: DAPI

Anti-Caspase 6 antibody [GT1154] used in Western Blot (WB). GTX01097

GTX01097 WB Image

WB analysis of various samples using GTX01097 Caspase 6 antibody [GT1154].
Dilution : 1:1000
Loading : 25 μg

Anti-Caspase 6 antibody [GT1154] used in Western Blot (WB). GTX01097

GTX01097 WB Image

Orthogonal

WB analysis of Jurkat cell extracts with/without Staurosporine (1μM, RT, 3 hrs) treatment using GTX01097 Caspase 6 antibody [GT1154].
Dilution : 1:1000
Loading : 25 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399