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Caspase 6 antibody

Anti-Caspase 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31707
Anti-Caspase 6 antibody used in Western Blot (WB). GTX31707

Cat. No. GTX31707

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Caspase 6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)

Product Note

Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Caspase-6 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Caspase-6.The immunogen is located within the first 50 amino acids of Caspase-6.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 6 , MCH2

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Caspase 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31707

GTX31707 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31707 Caspase 6 antibody.
Working concentration : 2 μg/ml

Anti-Caspase 6 antibody used in Western Blot (WB). GTX31707

GTX31707 WB Image

WB analysis of Jurkat cell lysate using GTX31707 Caspase 6 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399