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Caspase 7 antibody, N-term

Anti-Caspase 7 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX42546
Anti-Caspase 7 antibody, N-term used in Western Blot (WB). GTX42546

Cat. No. GTX42546

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all Caspase 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5μg/ml
IHC-P 10μg/ml

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

This antibody recognizes the N-Terminal region of Caspase 7.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A 16 amino acid peptide from human Caspase-7 amino-terminus.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 7 , CASP-7 , CMH-1 , ICE-LAP3 , LICE2 , MCH3

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-Caspase 7 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX42546

GTX42546 IHC-P Image

IHC-P analysis of rat skeletal muscle tissue using GTX42546 Caspase 7 antibody, N-term.

Anti-Caspase 7 antibody, N-term used in Western Blot (WB). GTX42546

GTX42546 WB Image

WB analysis of mouse skeletal muscle tissue lysate using GTX42546 Caspase 7 antibody at (A)0.5 and (B)1 ug/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409