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Caspase 7 antibody [SD20-57]

Recombinant antibody
Anti-Caspase 7 antibody [SD20-57] used in Western Blot (WB). GTX01057
Anti-Caspase 7 antibody [SD20-57] used in IHC (Paraffin sections) (IHC-P). GTX01057
Anti-Caspase 7 antibody [SD20-57] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01057
Anti-Caspase 7 antibody [SD20-57] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01057

Cat No. GTX01057

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 SD20-57

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($369)
See all Caspase 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 7 , CASP7 , CMH1 , ICELAP3 , LICE2 , MCH3 , Caspase 7

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-Caspase 7 antibody [SD20-57] used in Western Blot (WB). GTX01057

GTX01057 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody [SD20-57] (GTX01057) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 7 antibody [SD20-57] used in IHC (Paraffin sections) (IHC-P). GTX01057

GTX01057 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01057 Caspase 7 antibody [SD20-57].

Anti-Caspase 7 antibody [SD20-57] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01057

GTX01057 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01057 Caspase 7 antibody [SD20-57].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Caspase 7 antibody [SD20-57] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01057

GTX01057 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX01057 Caspase 7 antibody [SD20-57].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369