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Caspase 7 antibody

KO/KD Orthogonal IP/MS
Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
Anti-Caspase 7 antibody used in IHC (Paraffin sections) (IHC-P). GTX123679
Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679
Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679

Cat. No. GTX123679

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Caspase 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurakt (10 μM etoposide treatment for 24 hr)

Product Note

IP/MS validation was supported by references (PMID:30377415)

Predict Reactivity

Mouse, Zebrafish, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Caspase 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171013

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 7 , CASP-7 , CMH-1 , ICE-LAP3 , LICE2 , MCH3

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679

GTX123679 WB Image

KO/KD

Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679

GTX123679 WB Image

Orthogonal

Untreated (–) and treated (+) Jurakt whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Caspase 7 antibody used in IHC (Paraffin sections) (IHC-P). GTX123679

GTX123679 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Caspase 7 p11 subunit(GTX123679) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679

GTX123679 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 7 antibody used in Western Blot (WB). GTX123679

GTX123679 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jan MW et al. Front Immunol 2021; 12 : 753683 Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus Encephalitis.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169