GeneTex
United States (US)

Caspase 7 antibody

KO/KD Orthogonal IP/MS
GTX123679_40751_20170703_WB_KO_watermark_18121410_362.jpg
GTX123679_40751_20170814_WB_Etoposide_18121410_943.jpg
GTX123679_40751_IHC_18121410_965.jpg
GTX123679_40751_20170703_WB_18121410_832.jpg

Cat No. GTX123679

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurakt , Jurakt (10 μM etoposide treatment for 24 hr)

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377415)

Predict Reactivity

Mouse, Rat, Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Caspase 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 7,Casp-7,Cmh-1,Ice-Lap3,Lice2,Mch3,Casp7

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX123679_40751_20170703_WB_KO_watermark_18121410_362.jpg

GTX123679 WB Image

KO/KD

Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX123679_40751_20170814_WB_Etoposide_18121410_943.jpg

GTX123679 WB Image

Orthogonal

Untreated (–) and treated (+) Jurakt whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX123679_40751_IHC_18121410_965.jpg

GTX123679 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Caspase 7 p11 subunit(GTX123679) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123679_40751_20170703_WB_18121410_832.jpg

GTX123679 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169