*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2 , Jurakt , Jurakt (10 μM etoposide treatment for 24 hr)
IP/MS validation was supported by references (PMID:30377415)
Mouse, Rat, Zebrafish, Xenopus laevis(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.93 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the C-terminus region of human Caspase 7. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq]
GTX123679 WB Image
Untreated (–) and treated (+) Jurakt whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX123679 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Caspase 7 p11 subunit(GTX123679) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX123679 WB Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.