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Caspase 7 antibody

Anti-Caspase 7 antibody used in Western Blot (WB). GTX31704
Anti-Caspase 7 antibody used in IHC (Paraffin sections) (IHC-P). GTX31704

Cat No. GTX31704

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Human Skeletal Muscle Tissue Lysate

Specificity/Sensitivity

Depending on cell lines or tissues used, other cleavage products may be observed.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Caspase-7 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-7.The immunogen is located within the last 50 amino acids of Caspase-7.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 7 , CASP-7 , CMH-1 , ICE-LAP3 , LICE2 , MCH3

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-Caspase 7 antibody used in Western Blot (WB). GTX31704

GTX31704 WB Image

WB analysis of human skeletal muscle cell lysate using GTX31704 Caspase 7 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-Caspase 7 antibody used in IHC (Paraffin sections) (IHC-P). GTX31704

GTX31704 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX31704 Caspase 7 antibody.
Working concentration : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339