APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human CASP8(AA : 302-464) expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510](/templates/default/js/lightbox/images/loading.gif "Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510")
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GTX60510 IHC-P Image
IHC-P analysis of colon cancer tissue using GTX60510 Caspase 8 antibody [1H11].
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GTX60510 ELISA Image
ELISA analysis of antigen using GTX60510 Caspase 8 antibody [1H11].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60510 WB Image
WB analysis of HeLa (1), Jurkat (2), THP-1 (3), NIH3T3 (4), Cos7 (5) and PC-12 (6) cell lysate using GTX60510 Caspase 8 antibody [1H11].
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GTX60510 FACS Image
FACS analysis of NIH3T3 cells using GTX60510 Caspase 8 antibody [1H11].
Blue : Caspase 8
Red : negative control
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GTX60510 IHC-P Image
IHC-P analysis of liver cancer tissue using GTX60510 Caspase 8 antibody [1H11].
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REFERENCE
There are currently no references for Caspase 8 antibody [1H11] (GTX60510). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Caspase 8 antibody [1H11] (GTX60510). Be the first to share your experience with this product.
![Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510](/upload/website/prouct_img/small/GTX60510/GTX60510_20170912_IHC-P_w_23061123_474.webp "Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in ELISA (ELISA). GTX60510](/upload/website/prouct_img/small/GTX60510/GTX60510_20170912_ELISA_w_23061123_161.webp "Anti-Caspase 8 antibody [1H11] used in ELISA (ELISA). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in Western Blot (WB). GTX60510](/upload/website/prouct_img/small/GTX60510/GTX60510_20170912_WB_w_23061123_438.webp "Anti-Caspase 8 antibody [1H11] used in Western Blot (WB). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in Flow cytometry (FACS). GTX60510](/upload/website/prouct_img/small/GTX60510/GTX60510_20170912_FACS_w_23061123_939.webp "Anti-Caspase 8 antibody [1H11] used in Flow cytometry (FACS). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510](/upload/website/prouct_img/small/GTX60510/GTX60510_20170912_IHC-P_1_w_23061123_224.webp "Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510](/upload/website/prouct_img/normal/GTX60510/GTX60510_20170912_IHC-P_w_23061123_474.webp "Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in ELISA (ELISA). GTX60510](/upload/website/prouct_img/normal/GTX60510/GTX60510_20170912_ELISA_w_23061123_161.webp "Anti-Caspase 8 antibody [1H11] used in ELISA (ELISA). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in Western Blot (WB). GTX60510](/upload/website/prouct_img/normal/GTX60510/GTX60510_20170912_WB_w_23061123_438.webp "Anti-Caspase 8 antibody [1H11] used in Western Blot (WB). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in Flow cytometry (FACS). GTX60510](/upload/website/prouct_img/normal/GTX60510/GTX60510_20170912_FACS_w_23061123_939.webp "Anti-Caspase 8 antibody [1H11] used in Flow cytometry (FACS). GTX60510")
![Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510](/upload/website/prouct_img/normal/GTX60510/GTX60510_20170912_IHC-P_1_w_23061123_224.webp "Anti-Caspase 8 antibody [1H11] used in IHC (Paraffin sections) (IHC-P). GTX60510")