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Caspase 8 antibody [2G12]

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in Western Blot (WB). GTX34167

Cat No. GTX34167

Host

Mouse

Clonality

Monoclonal

Clone Name

2G12

Isotype

IgG1

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
IHC-P 1:200-1:500
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ALPS2B,CAP4,CASP8,Casp8,FLICE,MACH,MCH5,caspase 8,Caspase 8

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

IHC-P analysis of human liver tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

IHC-P analysis of human colon tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX34167 Caspase 8 antibody [2G12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX34167 Caspase 8 antibody [2G12].

Anti-Caspase 8 antibody [2G12] used in Western Blot (WB). GTX34167

GTX34167 WB Image

WB analysis of various sample lysates using GTX34167 Caspase 8 antibody [2G12].
Lane 1 : HeLa cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf