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Caspase 8 antibody [2G12]

Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167
Anti-Caspase 8 antibody [2G12] used in Western Blot (WB). GTX34167

Cat No. GTX34167

Host Mouse
Clonality Monoclonal
Clone Name 2G12
Isotype IgG1
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Specificity/Sensitivity

The antibody detects endogenous Caspase-8 protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 8,Alps2B,Cap4,Casp-8,Flice,Mach,Mch5,Casp8

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Caspase 8 antibody [2G12] used in IHC (Paraffin sections) (IHC-P). GTX34167

GTX34167 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Spleen Tissue using Caspase-8 Monoclonal Antibody.

Anti-Caspase 8 antibody [2G12] used in Western Blot (WB). GTX34167

GTX34167 WB Image

Western Blot (WB) analysis of 1) HeLa, 2) Mouse Brain Tissue, 3) Rat Brain Tissue using Caspase-8 Monoclonal Antibody.

Package List Price ($)
$ 319